percp conjugated miltenyi biotec rrid ab 2725959 cd8 apc vio770  (Miltenyi Biotec)

Journal: iScience

Article Title: Altered immunometabolic response to fasting in humans living with obesity

doi: 10.1016/j.isci.2025.112872

Figure Lengend Snippet: Cell counts and T cell subsets at baseline and after a 48-h fast in individuals with L-BMI or O-BMI (A) Total white blood cell count. (B) Absolute lymphocyte counts in whole blood. (C) Proportion of white bloods cells that were lymphocytes in whole blood. (D) Proportion of isolated T cells derived from whole blood that were CD4 + /CD8 − , CD4 − /CD8 + , or double-negative (CD4 − /CD8 − ). (E) Proportion of CD4 + T cells that were either Th1, Th2, Th17, Th22, or Treg cells. Large circles or triangles are estimated marginal means and black vertical bars are 95% confidence intervals derived from a linear mixed effects model. Orange circles represent the lean BMI (L-BMI) group and teal triangles represent the obese BMI (O-BMI) group. Small circles or triangles represent individual participant data. N = 32; 8 males/8 females per BMI group. ∗Group × time interaction, p < 0.05; ‡main effect of time, p < 0.05; †main effect of group, p < 0.05; c L-BMI vs. O-BMI at baseline, p < 0.05.

Article Snippet: Treg, CD4 + , and CD8 + cells were quantified at Baseline and 48 h. First, 1 × 10 6 freshly isolated T cells were stained with CD45 VioBlue (Miltenyi Biotec, Cat. No. 130-110-637), CD4 VioGreen (Miltenyi Biotec, Cat. No. 130-113-230), CD25 VioBright B515 (Miltenyi Biotec, Cat. No. 130-113-287), CD127 PE (Miltenyi Biotec, Cat. No. 130-133-753), and CD8 PerCP-Vio700 (Miltenyi Biotec, Cat. No. 130-110-682) for 10 min in the dark at 4°C.

Techniques: Cell Counting, Isolation, Derivative Assay

Journal: iScience

Article Title: Altered immunometabolic response to fasting in humans living with obesity

doi: 10.1016/j.isci.2025.112872

Figure Lengend Snippet: Markers of T cell function before and after a 48-h fast in individuals with L-BMI or O-BMI (A) Expression of CD4 on isolated human T cells. (B) Expression of CD8 on isolated human T cells. (C) Intracellular secretion of IFNγ in isolated human T cells following a 24-h activation culture. (D) Intracellular secretion of IL-17 in isolated human T cells following a 24-h activation culture. Large circles or triangles are estimated marginal means and black vertical bars are 95% confidence intervals derived from a linear mixed effects model. Orange circles represent the lean BMI (L-BMI) group and teal triangles represent the obese BMI (O-BMI) group. Small circles or triangles represent individual participant data. N = 32; 8 males/8 females per BMI group. ∗Group × time interaction, p < 0.05; ‡main effect of time, p < 0.05; †main effect of group, p < 0.05; c L-BMI vs. O-BMI at baseline, p < 0.05. MFI, median fluorescence intensity.

Article Snippet: Treg, CD4 + , and CD8 + cells were quantified at Baseline and 48 h. First, 1 × 10 6 freshly isolated T cells were stained with CD45 VioBlue (Miltenyi Biotec, Cat. No. 130-110-637), CD4 VioGreen (Miltenyi Biotec, Cat. No. 130-113-230), CD25 VioBright B515 (Miltenyi Biotec, Cat. No. 130-113-287), CD127 PE (Miltenyi Biotec, Cat. No. 130-133-753), and CD8 PerCP-Vio700 (Miltenyi Biotec, Cat. No. 130-110-682) for 10 min in the dark at 4°C.

Techniques: Cell Function Assay, Expressing, Isolation, Activation Assay, Derivative Assay, Fluorescence